GroEL/ES chaperonin system
In this example chain C of the GroEL/ES chaperonin system (PDB entry 3zpz) was fitted into the corresponding cryo-EM density map of the full complex (EMDB entry 2325 - 8.9 Å resolution) with a rotational sampling interval of 5 degrees. While Powerfit clearly favors one location based on cross correlation score and sigma difference, there is still a major break in sigma difference between the 7 symetric orientations and the next best fit (Fit 8).

Run 3zpz_C - EMD-2325 (Example3)
Status: FINISHED
Your PowerFit run has successfully completed.
Archive of the complete run: Example3.tgz
Archive of all autogenerated images: Example3_images.tgz
Please cite the following papers in your work:
G.C.P. van Zundert, M. Trellet, J. Schaarschmidt, Z. Kurkcuoglu, M. David, M. Verlato, A. Rosato and A.M.J.J. Bonvin.
The DisVis and PowerFit web servers: Explorative and Integrative Modeling of Biomolecular Complexes. J. Mol. Biol., Advanced Online Publication (2016).

G.C.P. van Zundert and A.M.J.J. Bonvin (2015)
Fast and sensitive rigid-body fitting into cryo-EM density maps with PowerFit.
AIMS Biophysics 2, 73-87.
and add the following acknowledgment:
The FP7 WeNMR (project# 261572), H2020 West-Life (project# 675858) and the EOSC-hub (project# 777536) European e-Infrastructure projects are acknowledged for the use of their web portals, which make use of the EGI infrastructure with the dedicated support of CESNET-MetaCloud, INFN-PADOVA, NCG-INGRID-PT, TW-NCHC, SURFsara and NIKHEF, and the additional support of the national GRID Initiatives of Belgium, France, Italy, Germany, the Netherlands, Poland, Portugal, Spain, UK, Taiwan and the US Open Science Grid.
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Solutions
The table below lists the 15 best non-redundant solutions found by correlation score. The first column shows the rank, column 2 the correlation score, column 3 the Fisher z-score column 4 the zscore as factor of standard deviations (z/σ), and column 5 the sigma difference to the best fit. (see N. Volkmann 2009, and Van Zundert and Bonvin 2016).
Rank (N) Cross Correlation Score Fisher z-score z-score/σ Sigma difference
(z1-zN)/σ
1 0.507 0.559 38.4 0.00
2 0.489 0.535 36.8 1.65
3 0.478 0.520 35.7 2.69
4 0.477 0.519 35.7 2.74
5 0.466 0.505 34.7 3.75
6 0.466 0.505 34.7 3.75
7 0.457 0.493 33.9 4.52
8 0.326 0.338 23.2 15.17
9 0.324 0.336 23.1 15.34
10 0.315 0.326 22.4 15.99
11 0.314 0.325 22.3 16.07
12 0.313 0.324 22.2 16.17
13 0.312 0.323 22.2 16.21
14 0.311 0.322 22.1 16.29
15 0.307 0.317 21.8 16.65
Images were generated with UCSF Chimera.
Fit 1
Rank 1
Cross Correlation Score 0.507
Fisher z-score 0.559
z-score/σ 38.4
Sigma difference (zN-zN+1)/σ 0.00
PDB Download
Fit 2
Rank 2
Cross Correlation Score 0.489
Fisher z-score 0.535
z-score/σ 36.8
Sigma difference (zN-zN+1)/σ 1.65
PDB Download
Fit 3
Rank 3
Cross Correlation Score 0.478
Fisher z-score 0.520
z-score/σ 35.7
Sigma difference (zN-zN+1)/σ 2.69
PDB Download
Fit 4
Rank 4
Cross Correlation Score 0.477
Fisher z-score 0.519
z-score/σ 35.7
Sigma difference (zN-zN+1)/σ 2.74
PDB Download
Fit 5
Rank 5
Cross Correlation Score 0.466
Fisher z-score 0.505
z-score/σ 34.7
Sigma difference (zN-zN+1)/σ 3.75
PDB Download
Fit 6
Rank 6
Cross Correlation Score 0.466
Fisher z-score 0.505
z-score/σ 34.7
Sigma difference (zN-zN+1)/σ 3.75
PDB Download
Fit 7
Rank 7
Cross Correlation Score 0.457
Fisher z-score 0.493
z-score/σ 33.9
Sigma difference (zN-zN+1)/σ 4.52
PDB Download
Fit 8
Rank 8
Cross Correlation Score 0.326
Fisher z-score 0.338
z-score/σ 23.2
Sigma difference (zN-zN+1)/σ 15.17
PDB Download
Fit 9
Rank 9
Cross Correlation Score 0.324
Fisher z-score 0.336
z-score/σ 23.1
Sigma difference (zN-zN+1)/σ 15.34
PDB Download
Fit 10
Rank 10
Cross Correlation Score 0.315
Fisher z-score 0.326
z-score/σ 22.4
Sigma difference (zN-zN+1)/σ 15.99
PDB Download